What is WST reagent?
WST-1 Assay Reagent ab155902 provides a simple, accurate and ready-to-use assay to measure cell proliferation, cell viability and cytotoxicity in mammalian cells. The WST-1 assay protocol is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases.
What does MTT reagent do?
The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. The darker the solution, the greater the number of viable, metabolically active cells. This non-radioactive, colorimetric assay system using MTT was first described by Mosmann, T et al.
What is a cell proliferation assay?
Cell proliferation assays typically detect changes in the number of cells in a division or changes in a cell population. Cell proliferation assays are mainly divided into four methods: metabolic activity assays, cell proliferation marker assays, ATP concentration assays, and DNA synthesis assays.
How do I analyze MTT results?
To calculate a viability assay like MTT, do the following:
- make an average of a few “empty” wells that contain your MTT solution but *no* cells.
- substract your background control from step 1 from all the measurements for this plate.
- calculate an average for your control (=healthy cells with 100% viability).
What is SRB blood draw?
Sulforhodamine B (SRB) Cellular Cytotoxicity Assay is one of the most widely used methods for detecting the cell viability or cytotoxicity of drugs. This test is based on the ability of SRB to bind cellular protein components and to measure total biomass.
How do you determine proliferation?
There are several methods available to measure cell proliferation rates. One method is to measure the overall metabolic activity inside a cell. Several dyes are available that can permeabilize a cell and react with certain enzymes and other factors and form a colored end-product which can be easily detected.
How do you calculate proliferation?
Proliferation index is calculated as the sum of the cells in all generations including the parental divided by the computed number of original parent cells theoretically present at the start of the experiment. It is a measure of the fold increase in cell number in the culture over the course of the experiment.
