What is the size of protein?
Eukaryotic proteins have an average size of 472 aa, whereas bacterial (320 aa) and archaeal (283 aa) proteins are significantly smaller (33-40% on average).
What is the molar mass of BSA?
66430.3 g/mol
Based on the amino acid sequence, the molecular weight of bovine serum albumin is 66430.3 daltons, which is equivalent to 66430.3 g/mol.
What is the size of albumin?
The glomerular pore size is equal to 4.0–4.5 nm,[5] and the molecular size of albumin is equal to 6.4 nm × 5.5 nm × 5 nm.
What is the size of an antibody in nanometers?
An antibody has a molecular weight of 150 kDa and a length of 10–15 nm, and the combination of a primary and a secondary antibody is up to 30 nm long.
Is BSA a glycoprotein?
BSA is described as a globular non-glycoprotein with a molecular weight close to 66 430. It is made of 583 amino acid residues and has 17 cystine residues (8 disulphides bridges and 1 free thiol group) (Carter and Ho 1994, Hirayama et al. 1990).
How do you make a 1 molar BSA?
A simple way of making a 1% BSA solution is to weigh out one gram of BSA powder, pour it into a graduated cylinder that can hold more than 100 milliliters (mL) of water, and then add water until the liquid level reaches the 100 mL mark. Mathematically, one divided by 100 equals 1%.
How many nanometers is an amino acid?
You can assume 3.5 Å per amino acid, so a 10 amino acid linear chain would be 3.5 nm approximately.
How big are the BSA and CA nanoparticles?
Bare BSA nanoparticles and calcium (Ca)-loaded BSA nanoparticles were fabricated at the targeted sizes, 100, 400, and 800 nm. The mean diameters of the prepared BSA nanoparticles were 125, 393, and 713 nm; those of the Ca-BSA nanoparticles were 260, 353, and 919 nm.
How is a 400 nm BSA particle prepared?
The 400 and 800 nm-sized BSA particles were prepared at pH 9 and 7, respectively, with 5% BSA content and 10 mM NaCl aqueous solution. If needed, calcium citrate tetrahydrate, a tenth of BSA content, was mixed under stirring at 500 rpm.
How does the pH affect the size of BSA?
The pH is the most important factor in controlling the coagulation of the BSA molecules during the desolvation process. The isoelectric point (pI) of BSA is about 4.9. When pH shifts toward the pI, the enhanced protein–protein interactions increase coagulation among BSA molecules; as a result, larger BSA particles could be formed.
How are bovine serum albumin ( BSA ) nanoparticles prepared?
BSA nanoparticles were prepared using a desolvation method with minor modifications ( Ko and Gunasekaran, 2006, Langer et al., 2003, Weber et al., 2000 ).
