What is the role of 4-Aminoantipyrine?

An anti-inflammatory drug that is not a steroid. In addition to anti-inflammatory actions, non-steroidal anti-inflammatory drugs have analgesic, antipyretic, and platelet-inhibitory actions….CHEBI:59026 – 4-aminoantipyrine.

What is the function of glucose oxidase?

Glucose oxidase enzymes are used to remove small amounts of oxygen from food products or glucose from diabetic drinks. Glucose oxidase is playing an important role in color development, flavor, texture, and increasing the shelf life of food products (Khurshid et al., 2011).

What is Trinder’s method?

The Trinder reaction is the reaction between hydrogen peroxide and the phenol and aminoantipyrine to form a quinone (quinoneimine), catalyzed by the presence of a peroxidase (such as horseradish peroxidase). The Stanbio Single Reagent Glucose Method is based upon the Trinder technique.

What is the principle of glucose oxidase?

Other uses of glucose oxidase include the removal of oxygen from food packaging and removal of D-glucose from egg white to prevent browning. PRINCIPLE: Glucose oxidase catalyses the oxidation of β-D-glucose to D-glucono- δ-lactone with the concurrent release of hydrogen peroxide (1).

What do phenol and 4 Aminoantipyrine combine with?

As outlined, the phenolic material was mixed with 4-aminoantipyrine in the presence of alkaline oxidising agent, preferable potassium ferricyanide, at high pH, formed a red quinone dye. Hence 4-aminoantipyrine was also known as Emerson’s reagent.

What was Aminopyrine used for?

Aminopyrine

What are oxidase enzymes?

Oxidases are enzymes that catalyze the oxidation of CN and CO bonds at the expense of molecular oxygen, which is reduced to hydrogen peroxide. The three principal substrates classes for oxidase enzymes are amino acids, amines, and alcohols.

What’s the meaning of peroxidase?

: an enzyme that catalyzes the oxidation of various substances by peroxides.

What is Trinder’s test?

The Trinder spot test is a diagnostic test used in medicine to determine exposure to salicylates, particularly to salicylic acid. The test employs the Trinder reagent (a.k.a. Trinder solution) which is mixed with a patient’s urine.

What is the hexokinase method?

A highly specific method for determining the concentration of glucose in serum or plasma by spectrophotometrically measuring the NADP formed from hexokinase-catalyzed transformations of glucose and various intermediates.

Why is glucose oxidase method specific?

Reagents are relatively inexpensive and the method should require a minimum of time, techniques and apparatus, be accurate and yield reproducible results. Glucose oxidase is an enzyme highly specific for glucose and is not react with blood saccharides. So it has been employed for the estimation of blood glucose.

How do you make phenol stock solution?

Take 100g phenol bottle to fume hood, open it, and pour in ~ 100 ml 50 mM TrisCl pH 8. Close lid tightly and shake gently. Leave to stand for an hour or two until the phenol liquifies and the phases are separated. Remove the supernatant with a pipette (dispose into the ‘chlorinated solvent waste’ container).

What is the role of 4-aminoantipyrine in the body?

It has a role as a non-steroidal anti-inflammatory drug, a non-narcotic analgesic, an antirheumatic drug, a peripheral nervous system drug, an EC 1.14.99.1 (prostaglandin-endoperoxide synthase) inhibitor, an antipyretic, a drug metabolite and a marine xenobiotic metabolite. It is a primary amino compound and a pyrazolone.

What is the molecular formula of quinoneimine?

Quinoneimine. PubChem CID. 102284669. Structure. Find Similar Structures. Molecular Formula. C23H27N3O8. Synonyms. quinoneimine.

How is the formation of quinoneimine dye measured?

The appearance of quinoneimine dye formed when coupled with 4-aminoantipyrine and phenol is measured at 500nm by spectrophotometry. One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute under the conditions described below.

How is 4-aminoantipyrine used in colorimetric determination of phenol?

4-aminoantipyrine for the colorimetric determination of phenolic materials was first proposed by Emerson in 1943. Hence 4-aminoantipyrine was also known as Emerson’s reagent. He proposed that the reaction consisted of mixing the phenolic material, 4-aminoantipyrine and an alkaline oxidant in a solution