What is the principle of the alkaline phosphatase test?
SUMMARY OF TEST PRINCIPLE AND CLINICAL RELEVANCE In the reaction, the ALP catalyzes the hydrolysis of the colorless organic phosphate ester substrate, p-Nitrophenylphosphate, to the yellow colored product p-Nitrophenol and phosphate. This reaction occurs at an alkaline pH of 10.3.
How do you prepare alkaline phosphatase?
You don’t need any special preparations for an alkaline phosphatase test. If your health care provider has ordered other blood tests, you may need to fast (not eat or drink) for several hours before the test. Your health care provider will let you know if there are any special instructions to follow.
What is the difference between acid phosphatase and alkaline phosphatase?
The actual pH varies with the particular substrate, but when phenyl phosphate is used, the acid phosphatases have an optimum pH of 4.9 and the alkaline phosphatases are optimally active at pH 9.8. In other organisms such as plants and fungi, acid phosphatases tend to predominate, and alkaline phosphatases are rare.
How do you turn off alkaline phosphatase?
Alkaline Phosphatase can generally be inactivated by heat: Heat the reaction for 10 min at +65 °C followed by at least one extraction with phenol/chloroform/isoamylalcohol (50 : 48 : 2).
What is alkaline phosphatase test used for?
The purpose of testing alkaline phosphatase is to determine if you have normal levels of this enzyme. An ALP blood test can be used for screening, diagnosis, and monitoring of liver diseases and other health conditions. Screening is checking for health problems before any symptoms have occurred.
Why alkaline phosphatase test is done?
What happens when serum alkaline phosphatase is high?
High levels Higher-than-normal levels of ALP in your blood may indicate a problem with your liver or gallbladder. This could include hepatitis, cirrhosis, liver cancer, gallstones, or a blockage in your bile ducts.
Which pH will have the highest alkaline phosphatase activity?
The pH optimum for alkaline phosphatase activity was 9.0 for all of the isolates tested. The pH optimum for acid phosphatase activity was 5.8 for 67% of the isolates and 4.8 for 33%. All of the isolates showed peak phosphatase activity at 37 degrees C.
