What is the meaning of transcriptome?
A transcriptome is the full range of messenger RNA, or mRNA, molecules expressed by an organism. The term “transcriptome” can also be used to describe the array of mRNA transcripts produced in a particular cell or tissue type.
What is the difference between genome and transcriptome?
In brief, the “genome” is the collection of all DNA present in the nucleus and the mitochondria of a somatic cell. The initial product of genome expression is the “transcriptome”, a collection of RNA molecules derived from those genes.
What is microarray Transcriptomics?
A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. The DNA molecules attached to each slide act as probes to detect gene expression, which is also known as the transcriptome or the set of messenger RNA (mRNA) transcripts expressed by a group of genes.
What is the transcriptome and how it is evaluated?
The concept of the transcriptome revolves around the complete set of transcripts present in a given cell type, tissue or organ and encompasses both coding and non-coding RNA molecules, although we often assume that it consists only of messenger RNAs (mRNAs) because of their importance in encoding proteins.
What is the use of transcriptomics?
Transcriptomics allows identification of genes and pathways that respond to and counteract biotic and abiotic environmental stresses. The non-targeted nature of transcriptomics allows the identification of novel transcriptional networks in complex systems.
What is the purpose of transcriptomics?
The key aims of transcriptomics are: to catalogue all species of transcript, including mRNAs, non-coding RNAs and small RNAs; to determine the transcriptional structure of genes, in terms of their start sites, 5′ and 3′ ends, splicing patterns and other post-transcriptional modifications; and to quantify the changing …
What is the transcriptome analysis?
The transcriptome is the complete set of transcripts in a specific type of cell or tissue. Generally, the goal of transcriptome analysis is to identify genes differentially expressed among different conditions, leading to a new understanding of the genes or pathways associated with the conditions.
What does the transcriptome contain?
The transcriptome encompasses all the ribonucleic acid (RNA) transcripts present in a given organism or experimental sample. RNA is the main carrier of genetic information that is responsible for the process of converting DNA into an organism’s phenotype.
What is transcriptome analysis used for?
What is the value of transcriptomics?
Transcriptome data obtained from different types of cells can help researchers to gain a deeper understanding of what constitutes a specific cell type, how that type of cell normally functions, and how changes in the normal level of gene activity may reflect or contribute to disease.
How are transcriptomes and metatranscriptomes of microbes used?
Next-generation RNA sequencing (RNA-Seq) of bacteria, viruses, and other microbes has become a standard method for analyzing transcriptome and metatranscriptome information. The transcriptome is the comprehensive set of RNAs encoded by an organism’s genome. Metatranscriptomes encompass all RNAs encoded by a group of organisms in a complex sample.
Where can I find the mouse transcriptome database?
The Mouse Transcriptome Project was an NIH-supported initiative that generated a free, public database of gene transcripts for many mouse tissues. These tissue-specific gene expression data, which are mapped to the mouse genome, are available in a searchable format in the Mouse Reference Transcriptome Database.
How is RNA seq used in bacterial transcriptomics?
Unlike hybridization-based methods such as microarrays, RNA-Seq enables unbiased strand-specific identification of common and novel transcripts. A wide dynamic range enables confident identification of both high and low expressors in a single bacterial, viral, or other microbial RNA-Seq experiment.
How are genes read out in a transcriptome?
For the instructions to be carried out, DNA must be “read” and transcribed – in other words, copied – into RNA ( ribonucleic acid). These gene readouts are called transcripts, and a transcriptome is a collection of all the gene readouts present in a cell.
