What is the function of NeuN?
NeuN (neuronal nuclei) antibodies are fundamental tools for staining mature neurons and studying neuronal development and differentiation.
What is anti NeuN?
NeuN is a reported synonym of the human protein ‘RNA binding fox-1 homolog 3’, encoded by the gene RBFOX3. The full protein is reported to be 312 amino acid residues in length. The NeuN antigen is a documented neuronal marker.
What is MAP2 staining?
The number of cells stained with an antibody raised against the microtubule-associated protein 2 (MAP2) is higher in homotopic than in heterotopic cocultures. This is true for both mesencephalic and striatal neurons. This effect is observed for both neuronal populations but is more pronounced in mesencephalic neurons.
What is TuJ1?
TuJ1 has been a marker of neurons in the central and peripheral nervous systems from the early stage of neural differentiation. FDCs expressed TuJ1 protein diffusely in both light and dark zones of germinal centers in all human lymphoid tissues.
Is MAP2 a neuronal marker?
MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast, tau is found predominantly in neuronal axons.
What kind of marker is MAP2?
Microtubule-associated protein 2 (MAP-2) is a dendritically enriched protein and marker of synaptic plasticity that has been observed to increase after TBI. Mondello et al.
What does TuJ1 stain for?
Tubulin beta III (TUBB3), also called TuJ1, is a tubulin thought to be involved specifically during differentiation of neuronal cell types [44]. Accordingly, immunohistochemical staining of TuJ1 is found in the cell bodies, dendrites, axons, and axonal terminations of immature neurons.
What is TuJ1 used for?
Why is the Nissl stain important?
This stain is commonly used to identify the neuronal structure in brain and spinal cord tissue. The Cresyl Violet method uses basic aniline dye to stain RNA blue, and is used to highlight important structural features of neurons.
Where does Neun bind in the cerebellum?
The Fox-3/NeuN antibody binds to the small cerebellum granule cell neurons, which form a prominent layer. In contrast antibody to the close relative of Fox-3/NeuN, Fox-2, is shown in red.
What kind of staining is used for neuronal cells?
Neuronal Nuclei (NeuN) is also known as Fox3. Staining for NeuN with clone 1B7 reveals strong nuclear staining of a wide range of neuronal cell types. There are some neuronal cells that are not detected by NeuN, such as Purkinje neurons, Golgi cells, and retinal photoreceptor cells.
How is Neun an antibody different from Nissl?
NeuN, an antibody derived from mice inoculated with purified neuronal nuclei, is specific to an unknown nuclear protein expressed by nearly all neurons and no glial cells. Unlike Nissl staining, this allows neurons to be visualized separately from glia.
What kind of neurons are not recognized by NeuN?
A few neuronal cell types are not recognized by NeuN antibodies, such as Purkinje cells, stellate and Golgi cells of the cerebellum, olfactory Mitral cells, retinal photoreceptors and spinal cord gamma motor neurons.
