What is meant by deep sequencing?

Deep sequencing refers to sequencing a genomic region multiple times, sometimes hundreds or even thousands of times. This next-generation sequencing (NGS) approach allows researchers to detect rare clonal types, cells, or microbes comprising as little as 1% of the original sample.

Can microarrays be used for sequencing?

For genomic analysis a tiling array design is desirable, in which DNA probes are chosen from contiguous stretches of the genome. Whereas only short-oligonucleotide microarrays are appropriate for detecting sequence changes, all types of microarray can be used to detect structural variation.

What is DNA sequencing services?

DNA sequencing by Sanger remains the gold standard in sequencing. Sanger is the technique of choice for projects like small construct/gene identification, SNP detection, microbial ID, and other applications which require a cost effective solution at a quick turnaround time.

What is deep targeted sequencing?

Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest. This method generally requires less sample input and produces a smaller amount of data than WGS, making analyses more manageable.

Is Sanger sequencing still used?

Sanger sequencing is still widely used for small-scale experiments and for “finishing” regions that can’t be easily sequenced by next-gen platforms (e.g. highly repetitive DNA), but most people see next-gen as the future of genomics.

Why is deep sequencing important?

Deep sequencing yields a unique genetic fingerprint that can be used to identify a person, and a trove of predictors of genetic medical diseases. Deep sequencing to identify epigenetic events including changes in DNA methylation and RNA expression can reveal the history and impact of environmental exposures.

Are microarrays obsolete?

Microarrays are reliable and more cost effective than RNA-Seq for gene expression profiling in model organisms. Microarrays will not become obsolete but might be relegated to only a few uses.

What companies make genetic sequencing machines?

Manufacturers of DNA sequencers

• Roche.
• Illumina.
• Life Technologies.
• Beckman Coulter.
• Pacific Biosciences.
• Oxford Nanopore.

What is a good sequencing depth?

In many cases 5 M – 15 M mapped reads are sufficient. You will be able to get a good snapshot of highly expressed genes. For that reason, many published human RNA-Seq experiments have been sequenced with a sequencing depth between 20 M – 50 M reads per sample.