What is Lipofectamine LTX?
Lipofectamine® LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest possible transfection efficiencies and viabilities.
What is Lipofectamine Plus reagent?
Lipofectamine LTX Reagent is a plasmid transfection reagent that offers a balance of potency and gentleness for your cells, resulting in high transfection efficiencies and viabilities.
What is plus reagent for in transfection?
PLUS™ Reagent is a proprietary reagent for pre-complexing DNA that enhances cationic lipid-mediated transfection of DNA into many cultured eukaryotic cells.
What is the transfection protocol for Lipofectamine LTX?
Lipofectamine® LTX & PLUS™ Reagent Protocol Outline A. Plate cells so they will be 70–90% confluent at the time of transfection. B. Prepare plasmid DNA-lipid complexes. C. Add DNA-lipid complexes to cells. Lipofectamine® LTX DNA Transfection Reagent Protocol See page 2 to view a typical plasmid transfection procedure. Transfection Amounts
What are the benefits of using HEK-293 cells?
The benefit of using HEK-293 cells for expressing recombinant proteins includes an efficient transfection of plasmid DNAs, faithful translation and processing of proteins [ 2 ].
When to culture HEK-293 cells for protein expression?
Animal cells and cell lines, such as HEK-293 cells, are commonly cultured at 37°C. These cells are often used to express recombinant proteins.
What happens to green fluorescent protein in HEK-293 cells?
As we demonstrate in this study, dropping culture temperature to 33°C, but not lower, 24 hours after transient transfection in HEK-293S cells will give rise to ~1.5-fold higher expression of green fluorescent protein (GFP) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors.