What is JM109 cells?
JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots).
What are XL1 Blue cells?
XL1-Blue cells are endonuclease (endA) deficient, which greatly improves the quality of miniprep. DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the. EcoK endonuclease system.
What is E coli JM101?
coli K12 JM101 is a recombination- and E. coli K12 restriction-proficient host strain. Intrinsic antibiotic resistance aids cell selection after a complete and successful transformation. For Research Use Only. Not for use in diagnostic procedures.
How do you read e coli genotype?
Genes: In E. coli, the genotype only includes the genes that carry a loss of function mutation. The gene name is listed as three-letters in lower case and italics (DNA methylase is written as dam). Different genes affecting the same function/pathway are identified with different uppercase italic letters.
What is BL21?
BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.
How do you read e coli genotypes?
What is the genotype of E. coli?
Genotypes of Invitrogen competent cells
| Bacterial strain | Genotype |
|---|---|
| DH10B | F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) nupG |
| DH10B T1R | F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) nupG tonA |
What are the different strains of E. coli?
These strains are:
- Shiga toxin-producing E. coli (STEC): This is the bacteria most commonly known for E.
- Enterotoxigenic E. coli (ETEC): This strain is commonly known as a cause of travelers’ diarrhea.
- Enteroaggregative E. coli (EAEC).
- Enteroinvasive E. coli (EIEC).
- Enteropathogenic E. coli (EPIC).
- Diffusely adherent E.
Does BL21 Express T7 polymerase?
The DE3 designation means that respective strains contain the λDE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promoter. Note that BL21 does not carry the gene for T7 RNA polymerase and thus is only suitable for expression from promoters recognized by the E.
Does BL21 have T7 polymerase?
BL21(DE3)pLysS is a derivative of BL21 that has the T7 RNA polymerase gene under the control of the lacUV5 promoter. This arrangement is on a phage genome, called DE3. DE3 is inserted into the chromosome of BL21 to make BL21(DE3).
What kind of gene does JM109 DE3 contain?
Bacterial Strain JM109 (DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109 (DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site.
What are the important features of JM109 strain?
JM109 is a K strain bacterium that provides minimized recombination and aids in plasmid stability which results in high quality plasmid DNA preparation. I can not tell the historical reasons, but you would see the merits by its genotype and its product sheets from companies.
Why is JM109 used for molecular cloning?
Why it is used for molecular cloning? Join ResearchGate to ask questions, get input, and advance your work. JM109 is a K strain bacterium that provides minimized recombination and aids in plasmid stability which results in high quality plasmid DNA preparation.
When to use JM109 for blue / white selection?
JM109 (DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109 (DE3) cannot be used for blue/white selection.
