What is HepG2 cell line?
Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. Hep G2 will respond to stimulation with human growth hormone.
How do you pass HepG2 passage?
Culture HepG2 in EMEM=10üS to 80% confluence on T75. Trypsinize (5ml) , add equal volume (5ml complete media) into falcon tube and spin 1000g for 5 minutes. Aspirate supernatant and re-suspend in 1ml of complete media.
How do you thaw HepG2 cells?
Thawing and Plating HepG2 Cells Remove the vial of cells from dry ice or storage unit. Defrost the vial of cells in a 37oC water bath with constant, moderate agitation, until ice in the ampoule is no longer visible. 2. Continue to warm the ampoule in the water bath for 30 seconds with gentle agitation.
What are HepG2 cells used for?
HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions.
Are HepG2 cells adherent?
HepG2 cells have adherent properties and grow as monolayers in small aggregates. HepG2 can be grown successfully at a large scale and stimulated with human growth hormone. They are also capable of secreting many plasma proteins, such as transferrin, fibrinogen, plasminogen and albumin.
What is Vero cell culture?
Vero cells are a lineage of cells used in cell cultures. The ‘Vero’ lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species).
What is the difference between Emem and Dmem?
The key difference between two media is the nutrient composition. EMEM is composed of the minimum concentrations of nutrients required for the growth of the culture, whereas DMEM is a much complex media with increased concentrations of amino acids and vitamins.
How do you freeze down a cell?
Freeze the cells slowly by reducing the temperature at approximately 1°C per minute using a controlled rate cryo-freezer or a cryo-freezing container such as “Mr. Frosty,” available from Thermo Scientific Nalgene labware (Nalge Nunc). Always use the recommended freezing medium.
What’s the definition of thawing?
1a : to go from a frozen to a liquid state : melt. b : to become free of the effect (such as stiffness, numbness, or hardness) of cold as a result of exposure to warmth. 2 : to be warm enough to melt ice and snow —used with it in reference to the weather. 3 : to abandon aloofness, reserve, or hostility : unbend.
What are Huh7 cells?
Huh7 is a type of human liver cell line that may be grown in the laboratory for research purposes. Huh7 cells have been instrumental in hepatitis C research. Until 2005, it was not possible to culture hepatitis C in the laboratory.
What is Vero cell?
green kidney
Vero cells are a lineage of cells used in cell cultures. The original cell line was named “Vero” after an abbreviation of verda reno, which means “green kidney” in Esperanto, while vero itself means “truth” in Esperanto.
Who Vero cell line?
Derived from the kidney of an African green monkey (Cercopithecus aethiops) in the 1960s, Vero cells are one of the most common mammalian continuous cell lines used in research.
Where does the HepG2 cell line come from?
HepG2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian male who had a well-differentiated hepatocellular carcinoma.
How is HepG2 used to treat hepatocellular carcinoma?
The HepG2 xenograft of human hepatocellular carcinoma (HCC) enables studies targeting antiangiogenesis (i.e. rapamycin, bevacizumab) or tumor growth inhibition (e.g. sorafenib). HepG2 cells have been demonstrated to be Neomycin G418 resistant (400 µg/mL).
How is the HepG2 transfection kit optimized?
HepG2 Transfection Reagent (0.5 ml / 1.5 ml / 8.0 ml) The transfection kit is optimized in HepG2 cells and enables a high efficiency transfer of siRNA, miRNA or plasmid DNA into the cells. The transfection reagent enables either a forward or reverse transfection method in order to achieve at least 90% transfection efficiency.
How long does it take to double a HepG2 cell?
Aspirate and add fresh culture medium every 2-3 days. HepG2 cell doubling time is 48 hours. To passage cells, rinse cell monolayer with 1x PBS twice and add pre-warmed (37°C) 0.05% Trypsin-EDTA solution to cover the bottom of the flask; incubate for 5 – 7 minutes.
