What is electrokinetic injection capillary electrophoresis?
There are two commonly used injection methods for CE: hydrodynamic and electrokinetic. Hydrodynamic injection is accomplished by the application of a pressure difference between the two ends of a capillary. Electrokinetic injection is performed by simply turning on the voltage for a certain period of time.
Which method is used in capillary electrophoresis?
Capillary zone electrophoresis (CZE) The capillary column is immersed into two buffer-filled reservoirs to which a high voltage is applied via platinum electrodes. The sample is stored in a separate reservoir and can be injected into the capillary by various techniques such as a hydrodynamic or electrokinetic impulse.
What are two main reasons why the resolution of capillary electrophoresis is usually higher than that of liquid chromatography?
If two ions are the same size, the one with greater charge will move the fastest. For ions of the same charge, the smaller particle has less friction and overall faster migration rate. Capillary electrophoresis is used most predominately because it gives faster results and provides high resolution separation.
How is apparent mobility calculated?
The apparent free electrophoretic mobility can be obtained by applying eqn [25] ( μ ( mm s − 1 ) = h × T max δ ) . The free electrophoretic mobilities of various marker proteins and five different mammalian carbonic anhydrases calculated by these procedures are listed in Table 6.
What does capillary electrophoresis detect?
Capillary electrophoresis (CE) is the primary methodology used for separating and detecting short tandem repeat (STR) alleles in forensic DNA laboratories worldwide. This chapter examines the general principles and components of injection, separation, and detection of STR alleles using CE.
What are the advantages of capillary electrophoresis?
Capillary electrophoresis (CE) provides a number of advantages for analysts, including high separation efficiency, short analysis times, low waste generation, and a diverse range of applications.
How does electroosmotic flow work?
Electroosmotic flow occurs when an applied driving voltage interacts with the net charge in the electrical double layer near the liquid/solid interface resulting in a local net body force that induces the bulk liquid motion.
Why is capillary electrophoresis used?
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. Capillary electrophoresis is used most predominately because it gives faster results and provides high resolution separation.
How does capillary electrophoresis differ from gel electrophoresis?
The key difference between capillary electrophoresis and gel electrophoresis is that gel electrophoresis is performed in a vertical or horizontal plane using a polymer gel of standard pore size whereas capillary electrophoresis is performed in a capillary tube with a polymer liquid or a gel.
How is electrophoresis achieved in pressure and electrokinetic injection?
This is achieved in either pressure or electrokinetic injections simply by suspending the sample in a buffer of lower conductivity ( e.g. lower salt concentration) than the running buffer.
What kind of electrophoresis is used in capillary separation?
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro-and nanofluidic channels.
Where is peak information stored in a capillary electrophoresis system?
Peak information is automatically stored for easy retrieval Components of a Capillary Electrophoresis System Laser Inlet Buffer Capillary filled with polymer solution 5-20 kV Outlet Buffer Sample tray Detection window
How is capillary electrophoresis different from HPLC and EOF?
Unlike HPLC, in capillary electrophoresis there is no mass transfer between phases. In addition, the flow profile in EOF-driven systems is flat, rather than the rounded laminar flow profile characteristic of the pressure-driven flow in chromatography columns as shown in figure 5.
