What is cytospin used for?

A cytocentrifuge, sometimes referred to as a cytospin, is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined.

What is CSF cytospin?

Description: Cytocentrifugation is a method of gentle and controlled centrifugation, that produces a monolayer of preserved, displayed cells (when present). These are flattened constructively on a slide, offering a more open nucleus and more visually interpretable nuclear material.

Why do you Centrifuge or cytospin a body fluid specimen?

Cytocentrifugation (also known as “cytospin”) provides the best method for both concentrating cells in body fluid samples and maintaining cellular morphology. This allows the cells to be concentrated for appropriate identification.

What is a cytospin smear?

Cytospin smears revealed more bacteria and demonstrated better leukocyte morphology than did unconcentrated or conventionally centrifuged samples of small volumes of infected body fluids, allowing early diagnosis of infection.

What is the cytospin technique?

The cytospin technique uses a high speed centrifuge to concentrate the cells on a slide in a uniform monolayer 6 mm in diameter. The monolayer distribution enhances the morphological appearance of the cells present. Allow the slides to dry in air for several minutes and then stain them with Wright-Giemsa stain.

What is cytospin in histopathology?

A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.

What stain is routinely used on cytospin body fluid slides?

Cytocentrifuge Preparation (Cytospin™) The slide is either immediately fixed in 95% alcohol for Papanicolaou staining or air-dried for the Diff-Quik (or other Romanowsky-type) stain.

What is cytology in histopathology?

What is cytology? Cytology is the microscopic examination of cell samples. These samples can be collected from any area of the body.

What is the difference between histopathology and cytology?

The key difference between histopathology and cytology is that histopathology is the study of diseased tissues using a microscope while cytology is the study of individual cells of the body.

How do you fix cytospin in a cell?

Simply re-suspend your cells in medium and add formaldehyde (1:10 producing a 4% solution of formalin). I recommend using ‘charged’ slides for your cytospins and I spin at 500rpm for 10 minutes. The remaining cell suspension can quite happily be stored in the fridge for 12 months+ and more cytospins made at any point.

How does the cytocentrifuge of the Shandon cytospin work?

Using centrifugal principles, the Shandon Cytospin deposits cells onto a clearly-defined area of a glass slide and allows for the absorption of the residual fluid into the sample chamber’s filter card (except Shandon EZ Megafunnel). Cytocentrifugation also constructively flattens cells for excellent nuclear presentation.

What kind of cytospin is used for urinary cytology?

Samples for conventional urinary cytology were centrifuged using the Thermo Scientific Shandon Cytospin (Shandon Scientific Ltd, Cheshire, UK). After this, 2 layers of cell smear samples were prepared.

How many cases of cytospin and ThinPrep were performed?

In 131 cases (65 processed by conventional cytospin and 66 processed by ThinPrep), a subsequent biopsy was performed. The authors reclassified these cases according to the Paris System and an analysis between the 2 methods with regard to bladder biopsies was performed.