What is cytospin CSF?

A cytocentrifuge, sometimes referred to as a cytospin, is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined.

How do you preserve a CSF sample?

Use a sterile syringe and needle to inoculate 0.5-1.0 ml of CSF into the T-I medium. The remaining CSF should be kept in the collection tube. It should not be refrigerated, but should be maintained at room temperature (20-25°C) before Gram staining and other tests.

How do you Centrifuge CSF?

If > 1 ml of CSF is available (i.e., if the specimen volume is sufficient for centrifugation), it must be centrifuged at a force sufficient to sediment the bacteria. Typically, centrifugation at 1000 x g for 10-15 minutes is sufficient to sediment bacteria.

What is CSF material?

Cerebrospinal fluid (CSF) is a clear, plasma-like fluid (an ultrafiltrate of plasma) that bathes the central nervous system (CNS). It occupies the central spinal canal, the ventricular system, and the subarachnoid space.

What is cytospin preparation?

Cytospin preparations A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.

What is cytospin and its function?

The Cytospin centrifuge is a special purpose instrument designed to deposit cells evenly onto a glass slide. The instrument when used correctly produces a monolayer cell deposition in a defined area of the slide using centrifugal force.

Why CSF sample should not be refrigerated?

unavoidable, CSF should NOT be refrigerated, since it is an excellent culture medium and fungi will continue to replicate at 25-30°C. Other body fluid specimens may be stored at 4°C overnight, if necessary before culturing.

How do you process CSF?

A spinal needle will be inserted. An opening pressure is sometimes taken. An abnormal pressure can suggest an infection or other problem. Once the needle is in position, the CSF pressure is measured and a sample of 1 to 10 milliliters (mL) of CSF is collected in 4 vials.

How do you perform a CSF analysis?

During a cisternal puncture, your doctor inserts a needle into the back of your skull. A ventricular shunt or drain can collect CSF from a tube that your doctor places in your brain. This is done to release high fluid pressure.

What is the normal composition of CSF?

The total volume of CSF in the adult is approximately 140 mL. CSF is produced at a rate of 0.2–0.7 mL per minute or 500–700 mL per day. The main function of the CSF is to reduce buoyancy of the brain….Table 1.

How does a cytospin work?

The cytospin process works by wicking fluid into a filter while fluid samples are spun into a central column and deposited in a mono-layer onto a defined area of a slide. This allows the cells to be concentrated for appropriate identification.

What are the techniques for cytospin cell resuspension?

Techniques in Cytology – Cytospin: Cytospin Cell Resuspension Solution. The tissue culture media and PBS provide a pH balanced liquid and ion replacement for the cell’s native environment. The BSA and serum provide a source of protein as a nutrient to keep the cells healthy and alive for a relatively short amount of time.

How does the centrifuge work in a Cytospin cell?

A cytocentrifuge spins cells at an angle, at low speeds, and accelerates and decelerates gradually. The fluid from the suspension is absorbed onto filter paper while the centrifuge is spinning. This allows the cells to adhere to the slide in a monolayer format.

How long does it take to do cytology on CSF?

CSF samples require immediate processing, ideally within 1 hour from collection. Upon centrifugation cytology is commonly assessed on May-Grunwald-Giemsa stains.

How long does it take for CSF sample to be processed?

Total and differential cell counts provide important first information across a spectrum of pathologic conditions involving the central nervous system and its coverings. CSF samples require immediate processing, ideally within 1 hour from collection.