WHAT is a BPG column?
BPG columns are glass chromatography columns designed to meet the needs of process development and biopharmaceutical manufacture; hygienic design and operation; easy, efficient packing and running with the single-screw adapter.
What is a chromatography column made of?
Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.
What is Hetp?
HETP is a measure of zone broadening; in general, the lower the HETP value, the narrower is the solute peak. HETP depends on various processes that occur during elution of a chromatography sample. A popular and simple expression for HETP takes the form: (11.108)
What can column chromatography be used for?
Column chromatography is one of the most important methods of separating (and purifying) solids and liquids. It is most often used on a small-scale (a few grams or mL of material), as the amount of chemical waste and time spent eluting the column increase as the amount of material increases.
How long do chromatography columns last?
A related question that we hear quite often is “what is the shelf life of a capillary GC column.” Once again, there are no right or wrong answers and GC columns usually don’t ship with an expiration date on them – but in general, a pretty good guideline guide line is to use the column within about two years to ensure …
What happens when column chromatography runs dry?
If you let the column run dry the silica will start to crack and you will get poor separation of your compounds. As you run the column, never let the level of solvent go below the level of the silica gel or you will get poor results.
What is packing height?
The height of a transfer unit (HTU) is a measure of the separation effectiveness of the particular packings for a particular separation process. Determination of the packed height can be based on either the gas-phase or the liquid-phase.
How long does column chromatography take?
The truth is that column chromatography should take no more than 15 min, including fraction collection and evaporation for scales ranging from 1 mg to 10 g. For particularly difficult separations for 4 or more products with close Rf’s for 2 or more compounds in the mixture, add 10 min to your total column time.
How often should you change a GC column?
What is the diameter of a BPG 300 column?
The 10 mm internal diameter is suitable for BPG 300 and 450 columns. For storage purposes, the 25 mm blind flange with a clamp and gasket can be used to seal off the column. A clamp and gasket, 6 or 10 mm internal diameter, are required to connect the 25 mm sanitary flanged inlet/outlet to either valves or tubing of the same type.
How big is a BPG column in cytiva?
The 2-way or 4-way valves with 6 mm internal diameter are suitable for BPG 100, 140, and 200 columns. The 10 mm internal diameter is suitable for BPG 300 and 450 columns. For storage purposes, the 25 mm blind flange with a clamp and gasket can be used to seal off the column.
What kind of valve do I need for a BPG 200 column?
Sanitary stainless steel valves of the appropriate inner diameter are recommended to prevent contamination of the packed bed. The 2-way or 4-way valves with 6 mm internal diameter are suitable for BPG 100, 140, and 200 columns. The 10 mm internal diameter is suitable for BPG 300 and 450 columns.
What kind of clamp do you need for a BPG column?
For storage purposes, the 25 mm blind flange with a clamp and gasket can be used to seal off the column. A clamp and gasket, 6 or 10 mm internal diameter, are required to connect the 25 mm sanitary flanged inlet/outlet to either valves or tubing of the same type. Preflanged tubing in 6 and 10 mm internal diameter is available.
