What does horseradish peroxidase cleave?

Peroxidase, a heme-containing oxidoreductase, is a commercially important enzyme which catalyses the reductive cleavage of hydrogen peroxide by an electron donor. In addition to biomedical applications, horseradish peroxidase is one of the enzymes with important environmental applications.

What is the function of horseradish peroxidase in ELISA?

One commonly used enzyme conjugate in ELISA is horseradish peroxidase. Horseradish peroxidase (HRP) is a 40,000 Dalton protein, which catalyzes the reduction of hydrogen peroxide (H2O2) to water (H2O) [1].

What is a peroxidase inhibitor?

The inhibitor, localized in the cytosol of the cell, acts on peroxidases and catalase of the same tissue (homologous action) and of other tissues or organisms (heterologous action). Besides peroxidases, the inositol phosphoglycan-like compound inhibits catalase, another heme enzyme.

What is the specific activity of the horseradish peroxidase extract?

From the calculated results, the activity of crude horseradish peroxidase extract was 6.3027 U/ml and specific activity of 0.8586 U/mg.

Why does luminol react with horseradish?

Here, we look at this reaction – and what horseradish has to do with it. The first of these additions is a strong oxidising agent such as hydrogen peroxide; this chemical is directly involved in the reaction with luminol.

Is horseradish peroxidase light sensitive?

Long shelf life: the enzyme horseradish peroxidase and alkaline phosphatase are relatively stable when stored appropriately. Because these enzymes are light insensitive, they are not affected by the light generated during chemiluminescence.

What is horseradish peroxidase HRP and what does it do?

Horseradish peroxidase (HRP) is an enzyme used to amplify signal in photometric assays by catalyzing the conversion of chromogenic or chemiluminescent substrates for the detection of targets such as proteins, carbohydrates, and nucleic acids.

What is a horseradish peroxidase assay?

Introduction. Horseradish peroxidase (HRP) is an enzyme that when in the presence of hydrogen peroxide, oxidizes a substrate (oxygen donor), resulting in a color change that is quantifiable. There are about 40 HRP isoenzymes.

What is an inhibitor of catalase?

IT is well known that the activity of catalase is greatly inhibited by very small concentrations of potassium cyanide, hydrogen sulphide and especially hydroxylamine.

What is a competitive inhibitor of catalase?

4-Methylpyrazole, EDTA, 1,10-phenantroline and aminotriasole are catalase competitive inhibitors and cimetidine is non-competitive inhibitor. 4-Methylpyrazole has shown higher affinity to the enzyme than aminotriasole.

Does luminol react with horseradish?