What are the essential requirements of BSL 3?
Prerequisites for the construction of BSL-3/ Assessment of proposed facility.
What does Biosafety Level 3 involve?
Biosafety level 3 (BSL-3) is applicable to clinical, diagnostic, teaching, research, or production facilities where work is performed with agents that may cause serious or potentially lethal disease through inhalation, to the personnel, and may contaminate the environment.
What is the difference between BSL 2 and BSL 3?
Biosafety Level 2 (BSL-2) is suitable for work involving agents that pose moderate hazards to personnel and the environment. A BSL-3 lab is designed to contain an inhalation exposure risk for personnel working in the lab as well as individuals outside the lab.
What precautions and protocols are used in biosafety level 3 and 4 labs to protect personnel and prevent release of pathogens?
More protective primary barriers are used in BSL 3 laboratories, including solid-front wraparound gowns, scrub suits or coveralls made of materials such as Tyvek® and respirators as necessary.
What is negative air pressure in BSL-3?
-12.5 Pa
A BSL3 lab shall have a physical separation from the access corridors by way of a vestibule (sas or anteroom) or airlock with self closing doors. Between your Sas 1 and Sas 2 you must have a negative pressure (at least -12.5 Pa) and preferably with interlocked doors.
What precautions are required for BSL?
Summary of laboratory facilities for BSL 1 – 4. Universal Precautions include frequent hand washing, no mouth pipetting, no food or drink in the lab and proper disposal of biohazardous/medical waste, as well as the use of engineering controls and personal protective equipment (PPE).
Are Labs positive or negative pressure?
Air pressure in laboratories and animal care rooms should be negative in relation to the corridor or adjacent non-laboratory areas. Rooms housing immunocompromised animals should be at a positive pressure with respect to adjoining areas.
What do you need to know about Biosafety Level 3?
Biosafety Level 3 (BSL-3) is the recommended containment for work with agents or toxins that may cause serious or potentially fatal disease through inhalation exposure. Work at BSL-3 requires enhanced facility design, operational controls and special practices,…
Who is the biosafety officer for BSL-3?
The Biosafety Officer (in collaboration with the Institutional Biosafety Committee) must approve the location and design of any BSL-3 facility, and has final authority to authorize the commencement of BSL-3 work. The Biosafety Level-3 facility design and operational procedures must be documented.
When to use high containment Biosafety Level 3?
High containment biosafety level 3 (BSL-3/ABSL-3) laboratories for animals and research must be certified for use before initial operation, and on an annual basis to ensure that critical system components (HVAC/exhaust fans, air valves, fan motors) are operating to maintain the design parameters of the laboratory.
How are Biosafety Levels used by the IBC?
The IBC uses the biosafety levels recommended by the CDC and NIH as the usual standards of containment to be set for work with a given biohazardous material. Containment requirements are subject to modification by the IBC at its discretion, depending on the circumstances presented by a specific project.
