Is topoisomerase a binding protein?

Topoisomerase binding protein (TopBP1) interacts with the C-terminal of topoisomerase II beta. TopBP1 is required for DNA replication. It binds double-stranded DNA breaks and nicks and plays a role in rescuing stalled DNA replication forks.

Does topoisomerase repair DNA?

Topoisomerase II (Top2) is a nuclear enzyme involved in several metabolic processes of DNA. The analysis by immunofluorescence of the DNA DSB repair response in synchronized human cell cultures revealed activation of DNA-PKcs throughout the cell cycle and Rad51 foci formation in S and late S/G2 cells.

What is gyrase in DNA replication?

DNA gyrase is an essential bacterial enzyme that catalyzes the ATP-dependent negative super-coiling of double-stranded closed-circular DNA. Gyrase belongs to a class of enzymes known as topoisomerases that are involved in the control of topological transitions of DNA.

What is the mechanism of action of etoposide?

Etoposide (VP-16). Its mechanism of action is to cause single-strand and double-strand breaks in DNA through interaction with DNA topoisomerase II, inducing arrest in the G2-phase of the cell cycle [8,21,154]. This activity is mediated through the formation of a stable complex with DNA and topoisomerase II.

Which description about prokaryotic versus eukaryotic DNA replication is not correct quizlet?

In prokaryotes, the DNA replication is unidirectional; in eukaryotes, the replication is bidirectional. Which of the following descriptions about prokaryotic versus eukaryotic DNA replication is not correct? It proposes that the two nucleotide strands unwind and each serves as a template for a new DNA molecule.

What is topoisomerase II?

showSearch. Type II topoisomerases are topoisomerases that cut both strands of the DNA helix simultaneously in order to manage DNA tangles and supercoils. They use the hydrolysis of ATP, unlike Type I topoisomerase. In this process, these enzymes change the linking number of circular DNA by ±2.

What is topoisomerase in DNA replication?

Topoisomerase also plays an important maintenance role during DNA replication. This enzyme prevents the DNA double helix ahead of the replication fork from getting too tightly wound as the DNA is opened up.

What does topoisomerase II do?

EC no. Type II topoisomerases are topoisomerases that cut both strands of the DNA helix simultaneously in order to manage DNA tangles and supercoils. They use the hydrolysis of ATP, unlike Type I topoisomerase. In this process, these enzymes change the linking number of circular DNA by ±2.

What is DNA etoposide?

Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death.

What is the role of Topoisomerase 2 binding protein 1?

TOPBP1 DNA topoisomerase II binding protein 1 [ (human)] Summary. This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.

Which is a repressor of the TOPBP1 protein?

TopBP1 is a c-Abl-interacting protein and a repressor for c-Abl expression hRad9/hHus1/hRad1 complex acts as a checkpoint sensor during S phase by rapidly localizing to sites of DNA damage and transducing checkpoint responses by facilitating proper localization of downstream checkpoint proteins, including TopBP1.

What is the role of TOPBP1 in DNA replication?

(HuGE Navigator) human TopBP1 is involved in the formation of the initiation complex of replication in human cells and is required for the recruitment of Cdc45 to origins of DNA replication Interaction between SPBP and TopBP1 was found to be mediated by the ePHD domain of SPBP and the BRCT6 domain of TopBP1.

How does TOPBP1 maintain genome integrity in mitosis?

TopBP1 maintains genome integrity in mitosis by controlling chromatin recruitment of SLX4 and by facilitating unscheduled DNA synthesis. Findings demonstrate that TopBP1 and ATR are able to inhibit the synthesis of rRNA and to activate nucleolar stress pathway.