Is BamHI affected by methylation?

BamHI cannot methylate this modified sequence. Methyltransferase/endonuclease pairs which differ in their sensitivity to non-canonical methylation can be exploited to generate rare DNA cleavage sites.

Where does the restriction enzyme bamh1 cut?

BamHI binds at the recognition sequence 5′-GGATCC-3′ , and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in “sticky ends” which are 4 b.p. long. In its unbound form, BamHI displays a central b sheet, which resides in between a helices .

What is BamHI HF?

BamHI has a High Fidelity version BamHI-HF® (NEB #R3136). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

Does the restriction enzyme BamHI produce sticky or blunt ends?

(1995). BamHI binds at the recognition sequence 5′-GGATCC-3′, and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in sticky ends which are 4 bp long. In its unbound form, BamHI displays a central b sheet, which resides in between α-helices.

Would a digestion with BamHI produce a blunt or a sticky end?

(1995). BamHI binds at the recognition sequence 5′-GGATCC-3′, and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in sticky ends which are 4 bp long.

What is H in Bam h1?

H stands for strain H of Bacillus amyloliquefaciens in BamHI restriction enzyme.

Which is the high fidelity version of BamHI?

BamHI has a High Fidelity version BamHI-HF ® ( NEB #R3136 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

Which is E.coli strain carries the BamHI gene?

A E. coli strain that carries the BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). One unit is defined as the amount of BamHI required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.. May exhibit star activity in NEBuffer r1.1, r2.1 and rCutSmart Buffers.

How is the BamHI gene used in Neb?

For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page. A E. coli strain that carries the BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). One unit is defined as the amount of BamHI required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl..