How does MOG cause EAE?
The pathophysiology of EAE is based on the reaction of the immune system against brain-specific antigens. This reaction induces inflammation and destruction of the antigen carrying structures, resulting in neurological and pathological features comparable which those observed in MS patients.
How do you induce EAE?
Active induction of EAE in C57BL/6 mice is achieved by a single immunization of 200 μg MOG35-55. Draw the emulsion into the 1-ml glass tuberculin syringe with 18-G needle, taking care not to introduce air bubbles, and then replace the 18-G needle with a 25-G needle for immunization.
How do you induce experimental autoimmune encephalomyelitis?
Experimental autoimmune neuritis (EAN) is an animal model for both acute and chronic inflammatory demyelinating polyneuropathy (i.e., GBS and CIDP, respectively). EAN can be generated in rats, mice, or rabbits, and is typically induced by injection of myelin proteins (P0, P2, PMP22, and MAG).
What is EAE model?
Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis. This model is produced by administering a myelin basic protein peptide (MBP) fragment that induces an autoimmune response directed to the myelin sheath surrounding motor neurons.
What is mog35 55 peptide?
MOG (35-55) is a myelin oligodendrocyte glycoprotein (MOG) 35-55 is a minor component of CNS myelin. Produces a relapsing-remitting neurological disease with extensive plaque-like demyelination, common to the manifestations of multiple sclerosis. Induces strong T and B cell responses and is highly encephalitogenic.
How do you score EAE?
These defects are quantified using a standard EAE scoring system on a 0–5 disease severity scale: 0, no disease; 1, loss of tail tone; 2, hind limb weakness; 3, hind limb paralysis; 4, hind limb paralysis and forelimb paralysis or weakness; and 5, moribund/death10.
How does EAE model work?
In this model, autoreactive T cells are actively induced by peripheral immunization (occurring in lymph nodes and spleen, ‘afferent compartment’) with antigen emulsified in an adjuvant (in EAE) or by infection with an as yet unidentified pathogen (in MS).
Is EAE a good model for MS?
Many elements of this cascade of events have been identified, tested or confirmed in EAE. From the pathogenesis point of view, therefore, EAE is a good model for studying MS mechanisms, even more so than for testing or developing drugs (Farooqi et al., 2010).
Is EAE similar to MS?
EAE is primarily used as an animal model of autoimmune inflammatory diseases of the CNS, and it resembles MS, the prototypical such disease, in many respects (Gold et al., 2006; Steinman and Zamvil, 2005; 2006; Farooqi et al., 2010).
What is EAE clinical score?
Typically, EAE is scored on scale 0 to 5. Most researchers also give mice “in-between” scores (i.e. 0.5, 1.5, 2.5, 3.5) when the clinical picture lies between two defined scores. The scoring method differs slightly depending on the stage of disease (onset/peak vs. recovery), for each individual mouse.
What does pertussis toxin do in EAE?
Pertussis toxin (PT) has been widely used to facilitate the induction of experimental autoimmune encephalomyelitis (EAE) in rodents. It has been suggested that this microbial product promotes EAE by opening up the blood-brain barrier and thereby facilitates the migration of pathogenic T cells to the CNS.
Is EAE a good model of MS?
How is eae induced by Mog / CFA used?
This model can be used with any of the standard EAE treatment regimens. When used with prophylactic treatment, EAE induced by MOG 35-55 /CFA in C57BL/6 mice has the largest therapeutic window and is sensitive to the broadest range of targets of any EAE model. We recommend it as an initial screen for compounds aimed at MS treatment.
Which is the best protocol for EAE induction?
Recommended protocol for use with: These kits are recommended for study of experimental autoimmune encephalomyelitis (EAE), including testing efficacy of potential therapeutics. Each kit is sufficient to induce EAE in 10 mice, and consists of:
How is eae induced in C57BL / 6 mice?
Chronic EAE develops in C57BL/6 mice after immunization with an emulsion of MOG (either MOG 35-55 or MOG 1-125) in CFA, followed by administration of pertussis toxin (PTX) in PBS. The emulsion provides antigen which initiates expansion and differentiation of MOG-specific autoimmune T cells.
How many mice are needed for EAE induction?
These kits are recommended for study of experimental autoimmune encephalomyelitis (EAE), including testing efficacy of potential therapeutics. Each kit is sufficient to induce EAE in 10 mice, and consists of: Antigen (MOG 35-55 or MOG 1-125) in emulsion with complete Freund’s adjuvant (three pre-filled syringes)
