Are HeLa cells BSL 2?

Risk summary These organisms pose a moderate health hazard. Centers for Disease Control and Prevention (CDC) guidelines (pdf) require BSL-2 containment for work with HeLa cell lines.

What are HeLa S3 cells?

HeLa S3 is a clonal derivative of the parent HeLa line (see ATCC CCL-2). S3 was cloned in 1955 by T.T. Puck, P.I. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. ATCC confirmed this cell line is positive for the presence of Papillomavirus viral DNA sequences via PCR.

What cell type are HeLa cells?

HeLa cells are a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken in 1951 from Henrietta Lacks, a patient who eventually died of her cancer.

What is the ATCC catalog number of HeLa ccl2?

ECACC General Cell Collection: HeLa

Is HeLa adherent?

Morphologically, HeLa cells are epithelial-like acquiring two unique cell morphologies one is similar to that of adherent cells and the other appeared with micro-carriers stirred under high shear condition changing from a sphere to the shape of fried egg (Masters, 2002).

Can I order HeLa cells?

Today, Skloot says, a vial of HeLa cells can be purchased online for about $250 a vial.

Are HeLa cells adherent or suspension?

HeLa-S3 cell morphology during adaptation to SFM: The adaptation process from SM to SFM alters cell morphology. In SM, the cells grew as an adherent monolayer (anchorgenous-dependent).

Are HeLa cells anchorage dependent?

Two genes, cyclin-dependent kinase like 3 (cdkl3) and cytochrome c oxidase subunit (cox15), were up-regulated in the faster growing, anchorage-independent (suspension) HeLa cells relative to the slower growing, anchorage-dependent (attached) HeLa cells.

What have the HeLa cells done?

Over the past several decades, this cell line has contributed to many medical breakthroughs, from research on the effects of zero gravity in outer space and the development of the polio vaccine, to the study of leukemia, the AIDS virus and cancer worldwide.

How many HeLa cells are there?

Over the course of 26 years, 600,000,000 HeLa cells have been produced in my laboratory each week, for a total of 800 billion cells. That is a lot of cells, but it’s nothing compared with the total number of cells – approximately 100 trillion – that make up a human.

What is HEp-2 cell?

The HEp-2 cell, a native protein array with hundreds of antigens, provides an ideal substrate for the detection of ANA1. The detection of ANA in human serum is an important screening tool for connective tissue diseases, and IIF is the reference method for ANA testing1.

Can You culture ctll-2 IL2 dependent T cells?

I’ve just started growing CTLL-2 IL2 dependent T cells in culture, and need quite high numbers. The ATCC recommendations for culture are to subculture actively growing cells BEFORE they reach 2×10-5 cells/ml, but for my purposes this doesn’t give me as many as I would like.

How are ctll cells used in conditioned media?

Description: CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media. Also Known As:

How long does it take for ctll-2 cells to grow?

CTLL-2. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 100,000 cells/ml, 5% CO2, 37°c.

How many cells per mL for ctll-2 cryopreservation?

For cryopreservation of CTLL-2, freeze at approximately 6 x 1,000,000 cells/ml in 90% FBS + 10% DMSO. References: Gillis S, Smith KA. 1977 Long term culture of tumour-specific cytotoxic T cells.