What kind of virus is VSV?

Indiana vesiculovirus, formerly Vesicular stomatitis Indiana virus (VSIV or VSV) is a virus in the family Rhabdoviridae; the well-known Rabies lyssavirus belongs to the same family….

What cells do VSV infect?

VSV infects a broad range of animals, including cattle, horses, and swine. The genome of the virus codes for five major proteins, glycoprotein (G), matrix protein (M), nucleoprotein (N), large protein (L), and phosphoprotein (P).

Can VSV infect humans?

Yes. Humans can become infected with VSV when handling infected ani- mals (direct contact). In affected people, vesicular sto- matitis causes a flu-like illness with symptoms of fever, muscle aches, headache and weakness.

Is VSV an adenovirus?

Three recombinant adenoviruses (rAd) were successfully constructed that expressed the VSV Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein (both serotypes of G [VSV-IN-G-NJ-G]) with potentiality to induce protective immunity.

Is VSV a DNA virus?

VSV is another single-stranded RNA virus of the rhabdoviridae family, which includes the rabies virus.

How is VSV classified?

VSVs are classified in the order Mononegavirales (non-segmented-negative-sense RNA viruses), family Rhabdoviridae, genus Vesiculovirus.

How does VSV enter the cell?

VSV enters the cell via the endocytic pathway and subsequently fuses with a cellular membrane within the acidic environment of the endosome. Many virions that attach to the cell surface are not internalized, and many of the internalized virions appear to be degraded by proteases and other enzymes (Matlin et al., 1982).

What is human VSV?

VSV is a zoonotic arbovirus that belongs to the family, Rhabdoviridae, the same family as the rabies viruses. VSV has an 11 kb genome that consists of a single strand of negative-sense RNA.

What is VSV G gene?

Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion system in which cells are marked by different fluorescent proteins.

What does VSV stand for?

VSV

How is VSV treated?

There are no specific antiviral agents. Treatment consists of supportive care including the prevention or treatment of secondary infections. A mild antiseptic mouthwash may reduce the pain associated with oral lesions. Nutritional support is provided if eating is impaired.

What does VSV G stand for?

VSV-G

What are the genes of the VSV virus?

VSV has long served as a prototypic nonsegmented negative-strand RNA virus (NNSRV), partly because of the small number of genes that are encoded by its 11-kb genome ( 1 ). These genes include the nucleocapsid protein (N), a phosphoprotein (P), a matrix protein (M), a glycoprotein (G), and a large polymerase protein (L).

What kind of protein is the VSV G protein?

Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion system in which cells are marked by different fluorescent proteins.

How is the VSIV L protein encoded in the genome?

The VSIV L protein is encoded by half the genome, and combines with the phosphoprotein to catalyze replication of the mRNA. The VSIV M protein is encoded by an mRNA that is 831 nucleotides long and translates to a 229 amino acid-protein.

What is the structure of the VSV nucleocapsid particle?

Structures of the nucleocapsid-like particles (NLPs) of VSV and rabies virus have recently been solved ( 4, 5 ), showing that the N protein has 2 lobes angled together to form a cavity for encapsidation of the genomic RNA. Each N monomer accommodates 9 bases of RNA.