What is af488?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.

Is af488 a FITC?

Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. In addition, Alexa Flour 488 is pH insensitive (over a broad pH range) and tends to be brighter compared to FITC on most instruments.

What Colour is Alexa Fluor 568?

Table 1. Properties of Alexa Fluor Dyes

Which Alexa Fluor is red?

Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.

What Alexa 532?

Alexa Fluor 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

Is FITC same as 488?

High-performance alternatives to fluorescein The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.

Is af488 brighter than FITC?

Molecular Probes™ Alexa Fluor® 488 dye—with nearly identical spectral properties and quantum yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.

What wavelength is FITC?

FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.

Is Phalloidin a fluorophore?

Phalloidin, a toxin isolated from the death cap mushroom, Amanita phalloides, binds to filamentous actin with high affinity, and this has made fluorophore-conjugated phalloidin a useful tool in cellular imaging.

What color is Cy3?

Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

Is Alexa Fluor 488 FITC?

Is FITC and fluorescein the same?

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color.

How much does af488 NHS ester Lumiprobe cost?

Your cart total is $0, and your order qualifies for free express shipping to , ZIP . AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

Which is a better dye Alexa Fluor or af488?

AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

What kind of dye is rhodamine 110 af488?

From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties.

Which is an isomerically pure 5-af488 NHS ester?

This product is an isomerically pure 5-AF488. This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules. ROX maleimide is a thiol reactive dye derivative based on isomerically bright rhodamine X fluorophore.