What media is used for HeLa cells?

Culture of HeLa cells HeLa cells are maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 0.11 g/liter sodium pyruvate, 2 mM l-glutamine, 4.5 g/liter glucose, 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin in a humidified incubator at 5% (v/v) CO2.

How do you differentiate C2C12 cells?

Differentiation of C2C12 cells is achieved by replacing GM to differentiation media, DM [DMEM—high glucose no sodium pyruvate (Gibco), 2% horse serum (Gibco), 1% glutamine (Gibco), 1% pen/strep (Gibco)]. After 24 h in DM, fused cells should be visible. DM should be changed every 48 h.

What are C2C12 cells used for?

C2C12 cells are used to study the differentiation of myoblasts, osteoblasts, and myogenesis, to express various target proteins, and to explore mechanistic biochemical pathways.

What media is used for animal cell culture?

Animal cells are cultured using a completely natural media, or an artificial media along with some of the natural products. 1. Body fluid such as plasma, serum, lymph, amniotic fluid and much more are used. These fluids used as animal cell culture media after testing for toxicity and sterility.

How was the HeLa cell culture?

Gey’s lab assistant Mary Kubicek used the roller-tube technique to place the cells into culture. It was observed that the cells grew robustly, doubling every 20–24 hours unlike previous specimens that died out. The cells were propagated by Gey shortly before Lacks died of her cancer in 1951.

What is myoblast in biology?

Myoblasts are the embryonic precursors of myocytes (also called muscle cells). Myoblasts differentiate into muscle cells through a process called myogenesis. During myogenesis, the myoblasts fuse into multi-nucleated myotubes, which later become the muscle fibers.

What are C2C12 myoblast cells where did they come from?

C2C12 is a subclone from a myoblast line established from normal adult C3H mouse leg muscle1. The original cell line, C2, was obtained by Yaffe and Saxel in 1977 by establishing primary cultures from the thigh muscle of 2 month old normal mice, 70 hours after crush injury2.

Which is most commonly used medium for animal tissue culture?

In animal cell culture media, fetal bovine serum is the most common supplement. In order to supply an optimal culture medium, it is employed as an economical supplement. Serum supplies carriers water-insoluble nutrients, protease inhibitors, hormones and growth factors and binds and neutralizes the toxic moieties.

Is the commonly used animal cell culture medium?

Fetal bovine serum is the most common supplement in animal cell culture media. It is used as a low-cost supplement to provide an optimal culture medium. These media are also referred to as ‘defined culture media’ since the components in these media are known.

What is HeLa culture?

HeLa (/ˈhiːlɑː/; also Hela or hela) is an immortal cell line used in scientific research. Cell biologist George Otto Gey found that they could be kept alive, and developed a cell line. Previously, cells cultured from other human cells would only survive for a few days. Cells from Lacks’s tumor behaved differently.

How are C2C12 cells cultured in a flask?

1. C2C12 cells are cultured in growth medium in collagen-coated 150 cm 2 polystyrene flasks at 37 °C in humidified atmosphere of 5% CO 2. CRITICAL STEP: To avoid premature myoblast differentiation, cells are kept at very low confluence (~ 40%) and propagated before they reach higher confluency. 2.

What is the role of miR-26a in C2C12?

In C2C12 skeletal muscle cells, miR-26a targets the Polycomb group (PcG) Ezh2 methyltransferase, a negative regulator of muscle differentiation [38], thus favoring myogenesis [67,68]. Up-regulation of miR-26a is evident at the latest stages of C2C12 cells differentiation, appearing only after several days of cell culture in differentiation medium.

Where did the C2C12 cell line come from?

C2C12 cells were generated by Blau, Chiu, and Webster (1983) as a subclone of the C2 cell line isolated by Yaffe and Saxel (1977). C2C12 cells were originally derived from satellite cells from the thigh muscle of a 2-month-old female C3H mouse donor 70 h after a crush injury.

How to grow C2C12 cells in warm growth media?

Dilute cells to required density (I like to plate 30-40%, which is ~100k cells/mL) in warm growth media in a 50mL falcon tube Plate as required (directly on tissue culture dish), and incubate at 37 degrees Celsius in 5% CO2