What are the steps in performing a QTL analysis?
- Select parents that differ for a trait.
- Screen the two parents for polymorphic marker loci.
- Generate recombinant inbred lines (can use F2-derived lines)
- Phenotype (screen in field)
- Do a separate ANOVA on the effect of each marker.
- Declare QTL where F-test is significant.
What do you understand by QTL mapping?
Quantitative trait locus (QTL) mapping is a genome-wide inference of the relationship between genotype at various genomic locations and phenotype for a set of quantitative traits in terms of the number, genomic positions, effects, and interaction of QTL.
How do you find QTLs?
The simplest approach for detecting QTLs is to analyze the data one marker at a time. If an association exists between a molecular marker genotype and trait value, a trait locus is likely to be near that marker locus. The advantage of single-marker mapping is that it works for any population structure.
What is the difference between GWAS and QTL mapping?
The basic difference between GWAS and QTL mapping is that GWAS studies the association between alleles and and a binary trait, such as being a sufferer of a disease, while QTL analysis deals with the contribution of a locus to variation in continuous trait like height.
What is the downside about creating QTLs for research?
QTL mapping give low resolution but give you high statistical power for detecting a QTL. The disadvantage is that you are limited to the genetic diversity present into the parents of your segregating population. You could think of using advanced intercrosses for increasing the resolution.
What is the purpose of QTL analysis?
QTL analysis allows researchers in fields as diverse as agriculture, evolution, and medicine to link certain complex phenotypes to specific regions of chromosomes. The goal of this process is to identify the action, interaction, number, and precise location of these regions.
What is QTL Slideshare?
QTL is a region of the genome that contain gene(s)associated with a quantitative trait. Allelic variation at a QTL region causes phenotypic variation in a quantitative trait. It is coined by Gelderman .
What is QTL Mcq?
QTL is the chromosomal site at which a group of genes affecting quantitative traits located. Explanation: QTL (quantitative trait loci) is the chromosomal site at which a group of genes affecting quantitative traits is located. Example: height, weight etc. 3.
What is simple interval mapping?
Simple interval mapping (SIM) is an improvement because it tests for QTL presence every 2 cM between each pair of adjacent markers. Thus, the most likely position of a QTL and the size of its effects are estimated more accurately than with single-factor analysis.
