What is Eukaryotic Promoter Database?
The Eukaryotic Promoter Database (EPD) is an annotated, non-redundant collection of eukaryotic Pol II promoters, for which the transcription start site has been determined experimentally. Access to promoter sequences is provided by pointers to positions in nucleotide sequence entries.
What is a eukaryotic promoter?
Promoter sequences define the direction of transcription and indicate which DNA strand will be transcribed; this strand is known as the sense strand. Many eukaryotic genes have a conserved promoter sequence called the TATA box, located 25 to 35 base pairs upstream of the transcription start site.
How do you cite a eukaryotic promoter database?
Please cite us using the following references: EPDnew database description: The eukaryotic promoter database in its 30th year: focus on non-vertebrate organisms. Dreos, R., Ambrosini, G., Groux, R., Périer, R., Bucher, P.
How are bacterial and eukaryotic promoters different?
Promoter binding is very different in bacteria compared to eukaryotes. Eukaryotes require a minimum of seven transcription factors in order for RNA polymerase II (a eukaryote-specific RNA polymerase) to bind to a promoter. Transcription is tightly controlled in both bacteria and eukaryotes.
What binds to the promoter in eukaryotic cells?
The promoter contains specific DNA sequences that are recognized by proteins known as transcription factors. These factors bind to the promoter sequences, recruiting RNA polymerase, the enzyme that synthesizes the RNA from the coding region of the gene.
How does operator enhancer work?
In the Operon Model, the operator is the gene segment to which a repressor binds. This prevents the RNA polymerase from transcribing certain genes in the operon unit. Enhancer DNA sequences bind transcription factors called enhancer-binding proteins which increase the rate of transcription.
What is the core promoter in eukaryotes?
RNA polymerase II (Pol II) core promoters are specialized DNA sequences at transcription start sites of protein-coding and non-coding genes that support the assembly of the transcription machinery and transcription initiation.
What is the role of the TATA box in eukaryotic promoters?
The TATA box is named for its conserved DNA sequence, which is most commonly TATAAA. Many eukaryotic genes have a conserved TATA box located 25-35 base pairs before the transcription start site of a gene. The TATA box is able to define the direction of transcription and also indicates the DNA strand to be read.
How are eukaryotic promoters similar and how are they different from prokaryotic promoters?
Eukaryotic promoters are the regulatory sequences that initiate the transcription of eukaryotic organisms. Prokaryotic promoters are the regulatory sequences that initiates the transcription of prokaryotic genes. Prokaryotic promoter consists of upstream elements, -10 element and -35 elements.
What is the most important difference in structure of promoter between prokaryotes and eukaryotes?
Prokaryotes only carry three promoter elements, -10, -35, and the UP elements, whereas eukaryotes carry a wide variety of promoter elements (4). In addition, the use of enhancers in eukaryotic transcription allows the promoter sites in remote location on the DNA to initiate transcription.
