What is the purpose of a HyperLadder?
HyperLadder 25bp is a ready-to-use molecular weight marker for size determination of DNA fragments. It is especially designed for short fragments such as apoptotic DNA oligonucleotides. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 25bp from the vial to the gel.
What is the DNA HyperLadder?
HyperLadder™ 100bp is a molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% agarose gels. The HyperLadder 100bp is also supplied with a 5x loading dye for loading of sample DNA.
What is a HyperLadder?
Product Description. HyperLadder™ 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% TAE or TBE agarose gels.
What is a HyperLadder gel electrophoresis?
Application Notes | www.bioline.com/hyperladder. Nucleic acid electrophoresis is a widely performed molecular biology technique and is used to separate, identify and purify nucleic acids based on the principle of charge migration.
How much should I load HyperLadder?
Vortex the tube and use 5 L per lane. There is no need to add loading buffer to the ladder. Use the 5x loading buffer for sample loading, under no circumstances should it be used for diluting the ladder. The blue dye in the loading buffer runs at approximately 4 bp on a 1% gel.
How does SYBR Safe stain work?
SYBR Safe is a cyanine dye used as a nucleic acid stain in molecular biology. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).
How long does SYBR Safe last?
SYBR safe has to be mixed 1/10,000 in the same buffer in which you made your gel and the gel can be soaked in it before or after it is run. If you are resolving small fragments, before is recommended so there is no time for diffusion after. The bath should be kept protected from the dark and is good for about 2 weeks.
Why is SYBR Safe used?
Less hazardous, sensitive DNA gel stain Invitrogen SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can be used with either blue-light or UV excitation.
How much SYBR is safe?
Dilute SYBR Safe DNA Gel Stain concentrate 10,000-fold in TAE or TBE buffer prior to use. For most minigels, 50 mL of 1X stain is sufficient (e.g., dilute 5 µL of concentrate with 50 mL buffer). For larger gels, increase volumes proportionally, ensuring that the entire gel is fully immersed during staining.
How do you use SYBR Safe?
Add sufficient SYBR™ Safe DNA gel stain to cover the gel. A 50 mL volume is sufficient for staining most standard minigels. To stain larger gels, increase the volume of staining solution in proportion to the increased gel volume, and ensure that the gel is fully immersed during staining. 1.2 Incubate for 30 minutes.
