What is SOC broth used for?

SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

What does SOC media stand for?

Super Optimal broth
SOC Medium, shorthand for Super Optimal broth with Catabolite repression, is a nutritionally rich bacterial culture medium. SOC medium is typically used to grow competent cells to maximize the efficiency of bacterial plasmid transformations.

What is the purpose of SOC medium?

SOC Medium is a rich medium used primarily to aid recovery of bacterial competent cells following transformation. Use of SOC medium improves the molecular uptake whilst stabilizing the cells rapidly and so maximizing the efficiency of competent cells.

How do you use SOC media?

1. Add 1 mL of room temperature SOC Media to competent cells immediately after electroporation or chemical transformation. 2. Incubate the cells with gentle shaking for 60 minutes at 37ºC.

What is SOC outgrowth medium?

Add SOC to freshly transformed E. coli cells and incubate for up to 1 hour before plating on selective media. For NEB 10-beta and NEB Stable Competent E. coli cells and incubate for up to 1 hour before plating on selective media. …

Does SOC medium expire?

Which is better? SOC medium can be stored at either 4°C or Room Temperature depending on how fast it will be used. Storing at Room Temperature is convenient and adequate for short term usage (weeks to a couple of months). For long term storage, we recommend storing at 4°C.

What is the difference between LB and TB medium?

1.2. TB. ‘Terrific broth’ is richer than LB and includes glycerol as an energy source, leading to faster growth and consequently a higher yield of bacteria per volume. It also contains potassium phosphates to lower the chance of cell death due to a drop in pH.

What makes a Super Optimal Broth SoC or sob?

Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids. Super Optimal broth with Catabolite repression ( SOC) is SOB with added glucose. (Figures in parentheses are the masses required to create 1 liter of medium)

What kind of growth medium is SoC used for?

SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

What kind of Broth does Escherichia coli use?

Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli.

Which is Super Optimal broth with added glucose?

SOC medium can also be used to regenerate Klebsiella oxytoca strains for the improved transformation efficiency. Super Optimal broth with Catabolite repression ( SOC) is SOB with added glucose.