What is the molecular weight of beta-actin?
approximately 42 kD
The molecular weight (MW) of beta-actin is approximately 42 kD.
What is the kDa of beta-actin?
42 kDa
Though beta-actin has a molecular weight of 42 kDa, degradation will produce 35–38 kDa, ~ 30 kDa, 15 kDa, and other typical fragments on a Western blot, of which 35-38 kDa fragments are the most common.
What is a loading control Western blot?
Western blotting is a commonly used method to determine specific protein levels in a sample (e.g. tissue homogenate, extract). Loading controls also indicate the proper transfer of proteins to the membrane during the western blotting process. …
How do you write beta-actin?
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins.
What is the function of beta-actin?
These proteins play important roles in determining cell shape and controlling cell movement (motility). Studies suggest that β-actin may also be involved in relaying chemical signals within cells.
How do you write beta actin?
What is beta actin antibody?
β-Actin Antibody detects endogenous levels of β-actin. Due to the high sequence identity between the actin isoforms, β-Actin Antibody may cross-react with other actin isoforms.
Where is beta actin found?
muscle cells
There are six types of actin; four are present only in muscle cells, where they are involved in the tensing of muscle fibers (muscle contraction). The other two actin proteins, β-actin and gamma (γ)-actin (produced from the ACTG1 gene), are found in cells throughout the body.
What is beta actin DNA?
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.
Why is B actin used as a control?
Beta-Actin (42 kDa) is commonly chosen as a loading control due to its general expression across all eukaryotic cell types. The expression levels of this protein do not vary drastically due to cellular treatment, which is another reason the protein makes a suitable control.
