Where does the protein PDGF-BB come from?

Background: PDGF-BB. Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum.

How long does it take to reconstitute PDGF-BB protein?

Reconstitute at 100 μg/mL in sterile 4 mM HCl. The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.

What is the role of BB and BA in lymphocytes?

Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex ( MHC class III) region on chromosome 6.

Where are BB and BA located on chromosome 6?

Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex ( MHC class III) region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction.

What is the specific activity of recombinant human PDGF-BB?

Raines, E.W. et al. (1985) Methods Enzymol. 109 :749. The ED 50 for this effect is 1.5-6 ng/mL. The specific activity of Recombinant Human PDGF-BB is approximately 1.9 x 10 6 IU/mg, which is calibrated against recombinant human PDGF-BB WHO International Standard (NIBSC code: 94/728).

Which is more potent PDGF BB or AB?

PDGF AA, BB, and AB are the isoforms studied more extensively in skeletal cells. They have similar biological actions, although PDGF BB is more potent than PDGF AA, and PDGF AB has intermediate activity ( Centrella et al., 1991 ). PDGF stimulates bone cell replication and DNA synthesis, both in intact calvariae and in isolated rat osteoblasts.

Are there different forms of the PDGF-R gene?

There are two forms of the PDGF-R, alpha and beta each encoded by a different gene. Depending on which growth factor is bound, PDGF-R homo- or heterodimerizes.