What is PMSF solution?
Alternate Names: PMSF Solution is also known as Phenylmethylsulfonyl fluoride solution. Application: PMSF Solution is a nonspecific, irreversible inhibitor of serine proteases and other enzymes, including arylsulfatase A and trypsin. CAS Number: 329-98-6. Molecular Weight: 174.19. Molecular Formula: C7H7FO2S.
What does PMSF dissolve in?
isopropanol
Use. PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better. A common final concentration of PMSF is 1mM.
Why is PMSF toxic?
Phenylmethanesulfonyl fluoride (PMSF) is an acutely toxic and corrosive compound. PMSF hydrolyzes upon exposure to water liberating a toxic, acidic gas which in contact with metal surfaces can generate flammable and/or explosive hydrogen gas. Forms hydrofluoric acid upon exposure to water, which is extremely toxic.
How do I prepare for PMSF?
To prepare a 100 mM solution, add 17.4 mg of PMSF per milliliter of isopropanol. Store at −20°C. PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C.
How long does PMSF last in solution?
~35 minutes
The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature.
Is PMSF a phosphatase inhibitor?
Phosphatase Inhibitor Cocktail is a mixture of 5 different phosphatase inhibitors. It inhibits many tyrosine phosphatases, acid phosphatases, and alkaline phosphatases. PMSF (100 mM) is used for inhibiting serine proteases, including tryptase, chymotrypsin, callicrein, subtilisin, and thrombin.
Does PMSF inhibit trypsin?
Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.
Why is PMSF used?
Acting as a non-specific inhibitor, PMSF is often used to protect samples against the digestive functions of proteases, such as trypsin, chymotrypsin, and thrombin, as well as other enzymes. It is also used when preparing protein extracts taken from cells and tissues.
What is the function of PMSF?
PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin.
What is PMSF for in lysis buffer?
Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation.
What does PMSF inhibit?
Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain. In addition to inhibiting serine proteases, PMSF will also inhibit cysteine proteases, like papain (reversible by DTT treatment), and mammalian acetylcholinesterase.
Does PMSF inhibit lysozyme?
It is fine to add PMSF to inhibit proteases, it won’t interfere with lysozyme.
What kind of solvent is used to prepare PMSF?
Thus stock solutions of PMSF are generally prepared in anhydrous organic solvents (e.g. 100% ethanol, or anhydrous isopropanol) prior to use in aqueous media. Noted general features and benefits of PMSF include the following: Inhibits serine proteases such as trypsin and chymotrypsin
How long can PMSF be stored in water?
PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better. A common final concentration of PMSF is 1mM. PMSF is extremely toxic, and preparation of solutions should be done very carefully.
Is there a non toxic alternative to PMSF?
AEBSF (4- [2-aminoethyl]-benzenesulfonylfluoride, hydrochloride or Pefabloc) is a non-toxic alternative. It is an irreversible serine protease inhibitor, and is used at the same concentration as PMSF, but is nontoxic and stable in aqueous buffer solutions.
What should be the final concentration of PMSF?
A common final concentration of PMSF is 1mM. PMSF is extremely toxic, and preparation of solutions should be done very carefully. PMSF is not stable in alkaline conditions, or in water. For disposal, add alkali in water and wait to dispose.
