Is laccase a peroxidase?
Both types of enzymes oxidize phenolic compounds and aromatic amines via one-electron oxidations, which creates radicals. Besides differences in the prosthetic groups, the laccases also differ from the peroxidases by generally having a lower oxidation potential (6, 14).
What is the meaning of laccase?
: a blue copper-containing oxidase occurring especially in the sap of lacquer trees and having an ability to produce lacquer from the sap and induce oxidation of certain polyhydroxy phenols (as urushiol)
Which amino acid is found in greatest abundance in the active site of a laccase?
Which amino acid is found in greatest abundance in the active site of a laccase? C. (His) . The answer to this question is C because the five-membered imidazole rings depicted in Figure 1 are associated with histidine, an amino acid whose side chain is frequently encountered at the active site of metalloenzymes.
What is the carbon source used in laccase production media?
Glucose, mannose, maltose, fructose, and lactose are the commonly used carbon sources. The excess glucose and sucrose reduce the production of laccase by obstructing the initiation.
What is extracellular laccase?
Laccases are the model enzymes for multicopper oxidases and participate in several applications such as bioremediation, biopulping, textile, and food industries. The molecular weights of partially purified laccase were 52 kDa for rice bran and 55 kDa for wheat bran.
What is the mechanism of the laccase catalysis?
3. Mechanism of Laccases. The laccase catalysis occurs due to the reduction of one oxygen molecule to water accompanied with the oxidation of one electron with a wide range of aromatic compounds which includes polyphenol [21], methoxy-substituted monophenols, and aromatic amines [14].
What kind of oxidase does laccase belong to?
Laccase belongs to the blue multicopper oxidases and participates in cross-linking of monomers, degradation of polymers, and ring cleavage of aromatic compounds. It is widely distributed in higher plants and fungi.
What are the factors that influence phenol oxidase and peroxidase?
Factors known to influence phenol oxidase and peroxidase activities in soils include the concentration of soluble phenolic compounds, the lignin content of plant litter, soil pH, and nitrogen availability. In response to these variables, oxidative and hydrolase activities tend to shift in opposite directions.
How does soil phenol oxidase affect organic matter accumulation?
Soils with high oxygen availability, pH and mineral activity tend to be substrate limited: high in situ oxidative activities limit soil organic matter accumulation. Soils with opposing characteristics are activity limited: low in situ oxidative activities promote soil organic matter storage.
