What sequencing method might be useful to investigate bacteria drug resistance in a patient?

Owing to improvements in sequencing technologies, microbial whole-genome sequencing (WGS) has emerged as a central tool to control antibiotic resistance. WGS has been used to develop novel antibiotics and diagnostic tests.

What can whole genome sequencing be used for?

Whole-genome sequencing (WGS) is a comprehensive method for analyzing entire genomes. Genomic information has been instrumental in identifying inherited disorders, characterizing the mutations that drive cancer progression, and tracking disease outbreaks.

How whole genome sequencing can be used for diagnosis?

Whole genome sequencing is a helpful diagnostic tool in which a physician examines the complete genetic code of the patient, looking for certain changes or variants in the code.

Who is Amr WGS?

Antimicrobial resistance (AMR) has been declared a global public health emergency by WHO. Low-income and middle-income countries (LMICs) bear the greatest burden of AMR infections. Whole-genome sequencing (WGS) has the potential to greatly enhance AMR surveillance at both the national and international level.

How do you Analyse whole genome sequencing data?

WGS generates a huge amount of data in the form of sequence reads. In order to interpret these data, analysis entails a multistep process using different software tools that line up the reads, look for variations in genetic codes, and compare them to reference genomes, among many other tasks.

How was whole genome sequencing done?

Scientists take bacterial cells from an agar plate and treat them with chemicals that break them open, releasing the DNA. The DNA is then purified. of known length, either by using enzymes “molecular scissors” or mechanical disruption.

What is antimicrobial resistance genes?

Bacteria develop resistance mechanisms by using instructions provided by their DNA. Often, resistance genes are found within plasmids, small pieces of DNA that carry genetic instructions from one germ to another. This means that some bacteria can share their DNA and make other germs become resistant.

What are AMR genes?

AMR genes refer to the genes implicated in or associated with the resistance to one or more antibiotics. The resistance may result from the presence or absence of a gene or specific mutions acquired spontaniously or through evolution over time.