What is DNA recognition sequence?
A recognition sequence is a DNA sequence to which a structural motif of a DNA-binding domain exhibits binding specificity. Recognition sequences are palindromes.
What DNA sequence does PstI recognize?
PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer.
What size is a DNA recognition sequence?
Recombinant DNA Recognition sites for specific enzymes range in size from 4 to 13 base pairs, and, for most restriction enzymes used in gene cloning, are palindromes; sequences that read in the 5′-to-3′ direction on one strand, are the same as those in the 5′-to-3′ direction on the opposite strand.
What is the DNA recognition sequence for BamHI in a 5 to 3 direction?
BamHI binds at the recognition sequence 5′-GGATCC-3′ , and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in “sticky ends” which are 4 b.p. long. In its unbound form, BamHI displays a central b sheet, which resides in between a helices .
What is recognition sequence explain in brief class 12?
The restriction enzymes cut DNA at specific base sequence, and these specific base sequence is known as the recognition sequence. Roman numbers following the names indicate the order in which the enzymes were isolated from that strain of bacteria.
What is the recognition sequence for Hind 2?
Hind II recognizes the sequence GTPy/PuAC and generates fragments with blunt ends (1). Compatible ends Hind II generates fragments with blunt ends and is compatible to any other blunt end.
What is CLA1?
CLA1, a gene previously isolated from Arabidopsis, encodes the first enzyme of the 2-C-methyl-d-erythritol-4-phosphate pathway, 1-deoxy-d-xylulose-5-phosphate synthase. Based on mRNA and protein expression patterns this enzyme is expressed mainly in developing photosynthetic and non-photosynthetic tissues.
What is the recognition sequence for EcoRI?
GAATTC
EcoRI recognizes the sequence GAATTC, and cuts both DNA strands between the G and the A nucleotides. Protruding from the cut ends will be single-stranded DNA “tails” having the sequences AATT.
What is the length of recognition sequence of Hind 2?
six base pairs
Hind-II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs.
How do you cut DNA sequence?
The discovery of enzymes that could cut and paste DNA made genetic engineering possible. Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends.
What is the sequence of the BclI recognition site?
BclI is a restriction endonuclease used in molecular biology applications to cut DNA at the recognition sequence 5′-T/GATCA-3′, resulting in fragments with 5′-cohesive termini.
Why the recognition sequences are palindromic?
Explanation: Enzymes such as restriction enzymes have to recognize a very specific sequence in order to carry out its task. It binds to the DNA only in one specific configuration. A palindromic sequence also increases the chance that both strands of DNA are cut.
