What are NHEK cells?

Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of adult skin (different locations) from a single donor and are provided in a cryopreserved format. They are the major cell type in the epidermis, making up about 90% of the cells.

What are the keratinocytes?

Keratinocytes are the most prominent cell within the epidermis. Keratinocytes are ectodermally derived and can be distinguished from melanocytes and Langerhans cells in the epidermis by their larger size, intercellular bridges, and ample cytoplasm. Keratinocytes are present in all four layers of the epidermis.

Where is keratinocytes found?

the epidermis
Keratinocytes are present in all four layers of the epidermis. Under physiological conditions, keratinocytes proliferate in the stratum basalis and over a 30–50-day period migrate through the epidermis to the stratum corneum. During this process, these cells undergo significant functional and morphological changes.

Are HEK293 immortal?

HEK293 is embryonic kidney immortalized cell line, which represents the epithelial morphology and possibility to form tumors in nude mice. HEK 293 cells are popular for their ease of growth and transfection, making them a common cell culture in cancer research.

How big are HEK293 cells?

What is the HEK293 Cell Size? HEK293 cell diameter is typically between 11 and 15µm, however, the diameter of cells is dependent upon their culturing conditions. Cells grown in an adherent monolayer culture will appear flatter and have a larger diameter than cells in suspension culture, which will be spheroid.

What type of cells are melanocytes?

Melanocytes. Melanocytes are dendritic, pigment-producing cells located in the basal cell layer (Figs 2.4, 2.5). They protect the skin from ultraviolet radiation. Individuals with little or no pigment develop marked sun damage and numerous skin cancers.