What is the difference between ITS1 and ITS2?
Conversely, there are two ITSs in eukaryotes: ITS1 is located between 18S and 5.8S rRNA genes, while ITS2 is between 5.8S and 28S (in opisthokonts, or 25S in plants) rRNA genes. ITS1 corresponds to the ITS in bacteria and archaea, while ITS2 originated as an insertion that interrupted the ancestral 23S rRNA gene.
What is ITS2 sequencing?
The internal transcribed spacer 2 (ITS2) has been used as a phylogenetic marker for more than two decades. It also provides several tools to process your own ITS2 sequences, including annotation, structural prediction, motif detection and BLAST14 search on the combined sequence-structure information.
What is ITS1 and ITS4?
ITS1 and ITS4 are general primers that amplifies the Internal Transcribed Spacer region for identification purpose. You can easily find a journal that provides the sequences for these primers: https://benthamopen.com/FULLTEXT/TOBIOTJ-14-70. Cite.
What are rRNA ITS sequences?
16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample.
What is the function of ITS2?
The ITS2 region unveiled a different ability to identify closely related species within different families and genera. The secondary structure of the ITS2 region could provide useful information for species identification and could be considered as a molecular morphological characteristic.
What is the use of ITS2?
ITS2 sequences have a relatively high divergence rate; thus, it can be used as a complementary locus to CO1 for identification of animal species. Recently, ITS2 region has been found to vary in primary sequences and secondary structures in a way that correlates highly with taxonomic classification.
What does ITS2 stand for?
Ribosomal internal transcribed spacer 2 (ITS2) exhibits a common core of secondary structure in vertebrates and yeast.
What is nuclear marker ITS2?
The ITS-2 of the nuclear rDNA as a molecular marker for populations, species, and phylogenetic relationships in Triatominae (Hemiptera: Reduviidae), vectors of Chagas disease.
How long is the ITS2 region?
Additionally, the significantly smaller size of ITS2 (average length of approximately 233 bp) compared with that of ITS (average length of approximately 634 bp) makes ITS2 a better candidate for barcoding studies. Identification efficiency of ITS and ITS2 by using BLAST.
What is ITS2 region?
The internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA is regarded as one of the candidate DNA barcodes because it possesses a number of valuable characteristics, such as the availability of conserved regions for designing universal primers, the ease of its amplification, and sufficient variability to …
