What is the difference between Hb electrophoresis and HPLC?

Haemoglobin electrophoresis provides qualitative analysis of Hbs (A, F, A2 and others), but high-performance liquid chromatography (HPLC) or capillary electrophoresis (CE) allows quantitation of the Hb fractions. If the aim is to detect β-thalassemia alone, Hb analysis by HPLC or CE (Fig.

What is the principle behind the Hb electrophoresis?

In the laboratory, a process called electrophoresis passes an electrical current through the hemoglobin in your blood sample. This causes the different types of hemoglobin to separate into different bands. Your blood sample is then compared to a healthy sample to determine which types of hemoglobin are present.

What is Hb variants estimation by HPLC?

Introduction: High-performance liquid chromatography (HPLC) is a method commonly used for the detection of hemoglobin (Hb) variants. This showed that rare Hb variants can have similar retention times to the five most common alpha or beta chain Hb variants.

What is HPLC electrophoresis?

Hemoglobin (Hb) Electrophoresis by HPLC test measures and identifies different types of hemoglobin in the blood. A human body contains various types of hemoglobin in their blood. With age, the percentage of hemoglobin present in the body changes. Hemoglobin is the protein which is present inside the red blood cells.

What is Hb HPLC blood test?

What is the use of HPLC?

HPLC is the form of liquid chromatography that is generally used in the pharmaceutical industry, as it can provide the precise results that are required. The results can be used to analyse finished drug products and their ingredients quantitatively and qualitatively during the manufacturing process.

What is the meaning of HPLC?

High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).

Which is better HPLC or electrophoresis for thalassemia?

Unlike electrophoresis, HPLC can identify and quantify low levels of Hb A 2 and Hb F, but comigration of Hb A 2 and Hb E occurs. Therefore HPLC is best used in the diagnosis of thalassemias rather than hemoglobinopathies because quantification of low levels of normal and abnormal hemoglobin levels is necessary to distinguish thalassemias.

Can you identify HB variants by HPLC alone?

However, the overlap of retention times of variants precludes definitive identification of Hb variants by HPLC alone. HPLC should not be used as the sole means of identification of Hb variants, particularly for laboratories that analyze only small numbers of samples. Table 1. Summary of Hb variant retention times by HPLC. . . . . Peak .

What kind of electrophoresis is used for hemoglobin analysis?

Various analytical schemes including electrophoretic and chromatographic methods have been developed for human hemoglobin analysis in a routine clinical laboratory. The most widely utilized method for hemoglobin analysis is the alkaline cellulose acetate electrophoresis at pH 8.6.

Where do hemoglobins migrate in an acid electrophoresis?

In an acid pH some hemoglobins assume a negative charge and migrate toward the anode, whereas others are positively charged and migrate toward the cathode (negative pole). For example, Hb S migrates with Hb D and Hb G on alkaline electrophoresis but separates from Hb D and Hb G on acid electrophoresis.